Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta-577 451, Karnataka, India
1Department of Biochemistry, Indian Institute of Science, Bengaluru-560012, Karnataka, India
2E.I. Dupont India Private Limited, Dupont knowledge center, ICICI Knowledge Park, Genome Valley, Hyderabad-500078, Telangana, India
In the present study, the genetic variations of leaf calli regenerants of Musa paradisiaca cv Karibale-Monthan has been analyzed using AFLP markers. The highest (86.66%) frequency of compact callus formation was obtained when leaf explants were cultured on MS media, containing 3mg/L 2, 4-D and 0.5 mg/L BAP. The optimum shoot formation and multiplication (5.70±1.49) was achieved from the leaf derived callus on MS medium fortified with 3mg/L BAP+0.5mg/L TDZ. The 3–4 cm small shoots produced distinct roots on MS media containing 0.5mg/l NAA with 0.2% activated charcoal. The AFLP analyses produced 1012 monomorphic bands from total 1094 bands with 7.5% polymorphism between mother plant and callus derived regenerants. The percentage of monomorphism for individual primer combinations was very high and varied from 90.3% (
AFLP, Callus, Genetic diversity, Indirect organogenesis, Karibale-Monthan
