1Department of Biotechnology, KL Deemed to be University, Vaddeswaram, Guntur-522 502, Andhra Pradesh, India
2Division of Crop Improvement, ICAR-Directorate of Floriculture Research, Pune-411 005, Maharashtra, India
3Tissue Culture Laboratory Division of Crop Improvement, ICAR-National Research Centre for Banana, Tiruchirapalli, 620 102, Tamil Nadu, India
4Tissue Culture Laboratory Division of Crop Improvement, ICAR-Indian Institute of Oil Palm Research, Pedavegi-534 475, West Godavari, Andhra Pradesh, India
Department of Biotechnology, KL Deemed to be University, Vaddeswaram, Guntur-522 502, Andhra Pradesh, India
*Corresponding author's e-mail: samuelsparjan@gmail.com
Online published on 7 May, 2019.
Oil palm (Elaeis guineensis Jacq.) has become the largest traded vegetable oil in the world due to its innumerable uses and its huge economic importance. Which increases the requirement of quality seedling but the conventional seed production limits the commercial seed production with its long germination period and low germination rate. In this context in vitro germination of zygotic embryo could be a promising alternative. Hence in this study the effect of culturing media, plant growth regulators and genotypes were assessed on in vitro regeneration and plantlet development of oil palm zygotic embryos. Zygotic embryos of four elite genotypes were cultured on MS, N6 and Y3 media, with or without plant growth regulators (NAA, BA& GA3-0.2mg/lt). There was no effect of culture media, plant growth regulators and genotypes on germination of zygotic embryos. Whereas on the growth and development of in vitro plantlets, there was a significant effect of culture media and genotypes. Where culture media N6 performs well and Y3 has shown more abnormality. In case of genotypes, genotypes G1 and G2 shown better performance when compared to genotypes G3 and G4. The effect of plant growth regulators on germination and plant growth was non significant.
Genotype, Germination, In vitro, Oil palm, Zygotic embryo
