1Agricultural-Technological Institute, RUDN University, Moscow, 117198, Russia
3Department of Plant Protection, Faculty of Agriculture, Sohag University, Sohag, 82524, Egypt
2All-Russian Research Institute of Plant Protection sh. Podbelskogo 3, St. Petersburg-Pushkin, 196608, Russia
The two-spotted spider mite is a destructive phytophagous of agricultural and horticultural crops. The disruptive selection had fulfilled on T. urticae females in 20 inbred generations. The selection of individual female genotypes was carried out according to a lethality rate of diagnostic concentrations of each acaricides. Malathion and fenpyroximate strains performed for comparative toxicity to five acaricides belonging to different chemical groups: dimethoate, bifenthrin, pyridaben, brompropylate and abamectin. The results allow us to conclude the malathion and fenpyroximate in mites with resistance alleles to one of these acaricides showed that, normalization of biochemical processes on the mitochondrial membrane depends on the function of the transport plasma membrane system. The expression of the malathion resistance gene was completely suppressed by the butyphos esterase inhibitor, while fenpyroximate resistance gene was not manifested in the inhibition of PBO activity of monooxygenases enzymes.
Acaricides, Carboxylesterase, Resistance, Synergists, Two-spider mites
