7, 8- Dihydroxyflavone, An Effective Natural Product Reduce Ralstonia solanacearum Populations and Control Tomato Bacterial Wilt

In this study, 7, 8- dihydroxyflavone (DHF) was isolated first time from medicinal plant Oroxylum indicum and its antibacterial potential against plant pathogen Ralstonia solanacerum was determined. The minimum inhibitory and bactericidal concentrations of DHF were 20 ìg/mL and 80 ìg/mL respectively. The biofilm formation and inhibition of DHF against R. solanacearum was investigated via scanning electron microscopy indicated that destruction of the cell wall. The inhibition of biofilm formation and molecular assays revealed that DHF binds lumen periplasm side of the outer membrane protein (EpsA) was identified. Moreover, DHF effectively reduced the bacterial wilt disease and control efficiency is as high as 89.10% at nine days after inoculation. Altogether, the antibacterial potential of DHF against R. solanacearum displayed effective agent can attenuates bacterial wilt disease in tomato plant.