1Department of Pomology, Agricultural and Biological Division. National Research Centre, El-Behouth St. 33, Cairo-12622, Egypt
2Department of Chemistry, Russian State Agrarian University-MTAA named after K.A. Timiryazev, 127550, Timiryazevsky proezd, 2, Moscow, Russian Federation.
*Corresponding Author: Esraa M.M. Farahat, Department of Pomology, Agricultural and Biological Division. National Research Centre, El-Behouth St. 33, Cairo-12622, Egypt, Email: emfarahat90@gmail.com
Online published on 19 August, 2021.
Humic substances plays a vital role in the plant tissue culture as a growth hormone for in vitro propagation of many plant seedlings. The aim of this study was to investigate the effect of added humic-fulvate complex (HFC) at the various concentrations on the growth and development of grape seedlings in in vitro at the different nutrient levels.
The cutting of khasansky grape were cultivated on 1/4 Murashige and Skoog medium or 1/2 Murashige and Skoog medium either alone or supplemented with the humic-fulvate complex at the different concentrations at (0.1, 1 and 10 ml/l). Then, they were cultured for 4 weeks under a controlled environment.
The data observed that the low concentration of Murashige and Skoog medium (1/4 MS) for in vitro rooting of grape cv. 'Khasansky ’either alone or combined with HFC at the various concentrations significantly increased the rooting percentages and the total length of roots and stimulating the rate of vegetative growth compared with cultivated in 1/2 MS medium either alone or with supplemented with HFC. 1/4 MS+ 10 ml/l HFC was the best treatment for improving the growth of khasansky grape seedlings.
Grape, Humic-fulvate complex (HFC), Root initiation, Tissue culture
