Estimation of biological toxicity by copper oxychloride on Pisum sativum L. and Vigna radiata L.

This research work describes about toxicity estimation of commonly used fungicide copper oxychloride using Pisum sativum L. and Vigna radiata L. as a bioindicator.

The seeds of P. sativum and V. radiata were treated with different concentrations of fungicide. Biological toxicity measured by seed germination percentage, R/P ratio, total leaf chlorophyll, total leaf proline, total seed protein, mitotic index, abnormality index and mitotic inhibition.

Linear regression analysis showed that seed germination percentage, total seed protein, abnormality index and mitotic inhibition show positive correlation with increasing concentration of fungicide copper oxychloride. The R/P ratio of 4^th and 8^th days, mitotic index, total leaf chlorophyll and leaf proline have negative correlation along increasing concentration of fungicide. However total leaf proline of V. radiata at 2% concentration of fungicide is abruptly higher than control and other concentrations. The application of copper oxychloride at lower concentration can be used as a safe fungicide.