1Institute of Biotechnology, Hue University, Provincial Road No. 10, Phu Vang, TTHue, Vietnam
2Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
3University of Science and Technology, Da Nang University, 54 Nguyen Luong Bang, Da Nang, Vietnam
4Hue University of Education, Hue University, 34 Le Loi, Hue, Vietnam
*Corresponding Author: Dang Thanh Long, Institute of Biotechnology, Hue University, Provincial Road No. 10, Phu Vang, TTHue, Vietnam, Email: dtlong@hueuni.edu.vn
Online Published on 15 July, 2022.
The DNA barcoding is currently an effective and widely used tool that enables rapid and accurate identification of plant species.
DNA barcoding of 9 chloroplast genes (rbcL, matK, trnH-psbA, accD-psal, ndhA, psbE-petL, Rpl32-trnL, trnW-psaJ, trnSGCU-trnGGCC) were used to provide the theoretical basis for species identification, genetic diversity analysis of lotus population collected in Thua Thien Hue province, Vietnam. Universal primers were used and sequence products were analyzed using the MEGA X program.
The results showed that high levels of haplotype diversity (Hd), ranging from 0.618–0.869 and low levels of nucleotide diversity (Pi), ranging from 0.180 x 10−3-3.280 x 10−3 base on a total of nine gene regions of chloroplast genome. The neutrality tests show an excess of rare nucleotide position variations in individuals' white lotus and derived haplotypes recent expansion. While the evolution of the individuals in the pink lotus may have to decrease. The phylogenetic analyses indicated that combined sequences were not insufficient to make a difference to the DNA barcoding in the individual's lotus of the N. nucifera species this is in the study. The standardized and accurate barcode information of lotus is provided for researchers. It lays the foundation for the conservation, evaluation, innovative utilization and protection of Nelumbonaceae germplasm resources.
Chloroplast, Genetic diversity, Lotus, N. nucifera
