Indian Journal of Agricultural Research
SCOPUSWeb of Science
  • Year: 2024
  • Volume: 58
  • Issue: 5

Influence of Storage Temperature and Duration on Phytochemical Contents and Antioxidant Activities of the Inflorescence of Aranda Hybrids

  • Author:
  • Jamnian Chompoo1, Oranee Chusuwan2, Supatida Abdullakasim2,*
  • Total Page Count: 8
  • Page Number: 820 to 827

1Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand

2Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand

*Corresponding Author: Supatida Abdullakasim, Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand, Email: fagrsds@ku.ac.th

Online Published on 14 November, 2024.

Abstract

Four Aranda hybrids, including Aranda Bangkhuntian Gold, A. Calypso, A. Royal Sapphire and A. Sayan Duangporn were analyzed for their bioactive substances and antioxidative activities as commercial cut flowers with suggested medicinal properties.

A completely randomized design was conducted for this study. Total flavonoids, total phenolics and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferrous ion (Fe (II)) radical scavenging activities were analyzed from the ethanoic extracts of four parts, including flower bud, blooming flowers, leaves and roots of the four Aranda cultivars. Furthermore, the dried powder samples were kept at room temperature (25-28°C) or cool (5-7°C) for six months to compare the bioactive substances and antioxidative activities after storage.

In most Aranda hybrids, the leaf ethanolic extracts had the highest total flavonoids and total phenolic contents range from 26.63-40.05 mg rutin equivalent (RUE)/g extract and 152.00-254.13 mg gallic acid equivalent (GAE)/g extract, respectively, which is remarkably different from the floral parts (bud or blooming flowers) that had only 8.00-20.80 mg RUE/g extract and 97.44-143.44 mg GAE/g extract, respectively. The highest ABTS and Fe (II) antioxidant activities were detected in the leaf part with 53.13-78.85% and 11.19-15.07%, respectively, while the inhibitory effect on DPPH of most Aranda hybrids was the highest in the root part (67.37-80.90%), which 1.13-2.78 folds higher than the floral parts. Compared among the four Aranda hybrids, the A. Bangkhuntian Gold had the highest leaf total phenolic content (291.67 mg GAE/g extract) and inhibitory activities on ABTS and DPPH in the root part, about 80.90-82.23%. At six months of dried sample storage at room temperature or in a refrigerator, the total phenolic contents, ABTS, DPPH and Fe (II) activities in all organs of most Aranda hybrids could maintain similar levels in both storage temperatures. In contrast, the total flavonoid contents of leaves and roots were more sensitive to room temperature since the level was markedly reduced.

Keywords

ABTS, DPPH, Flavonoid, Orchid, Phenolic