Indian Journal of Agricultural Research
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 60
  • Issue: 2

Identification and Molecular Characterization of S. dorsalis (Hood) in Blackgram using mtCOIII Marker

  • Author:
  • Rajasekhar Lella1*, Tirumalasetti Madhumati2, D.V. Sairam Kumar2, V. Prasanna Kumari3, V. Roja4
  • Total Page Count: 11
  • Page Number: 271 to 281

1Department of Agriculture, Government of Andhra Pradesh, India.

2Department of Entomology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India.

3Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India.

4Department of Biotechnology, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur-522 034, Andhra Pradesh, India.

*Corresponding Author: Rajasekhar Lella, Department of Agriculture, Government of Andhra Pradesh, India. Email: rajagrico724@gmail.com

Abstract

Identification and characterization of Scirtothrips dorsalis in blackgram.

Pictorial taxonomic key-based morphology identification of Scirtothrips dorsalis and molecular characterization through DNA sequencing using species specific marker mtCOIII for accurate confirmation of the species.

The present paper reports the host record of S. dorsalis on blackgram and its identification, molecular characterization through DNA barcodes from A.P. This study contributed 07 (seven) novel gene sequences to NCBI database and also revealed the existence of low genetic polymorphism among the mtCOIII sequences of S. dorsalis. Species specific mtCOIII primer was found promising. Some more genera of thrips were also identified viz. Megalurothrips usitatus, M. typicus (Bagnall), Ayyaria chaetophora (Karny), Phibalothrips peringueyi (Faure) and some Tubuliferan thrips in meager number found in all GBNV hotspots of blackgram across A.P.

Keywords

DNA barcodes, Genetic polymorphism, mtCOIII, S. dorsalis