1Department of Plant Pathology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior-474 004, Madhya Pradesh, India.
*Corresponding Author: Bhagyashree Singh, Department of Plant Pathology, Sai Nath University, Ranchi-835 219, Jharkhand, India. Email: juhisinghnduat@gmail.com
Alternaria blight is most destructive fungal disease of oilseed which cause severe losses 47% in seed quality and quantity. The current research aimed to identify the specific pathogen species and its aggressiveness prevailing in M.P. area.
Isolates of A. brassicae from five districts viz., Gwalior-A1, Morena-A2, Bhind-A3, Datia-A4 and Sheopur-A5 were cultured on potato dextrose agar medium to study their cultural characteristic and genetically variability in 7 days old culture. Genomic DNA from different fungal isolate culture having concentration of DNA to ~25 ng/μl was used for amplification of RAPD primers.
The distance matrix analysis was drawn phylogenetic tree by PCR data showed genetic distance among the fungal isolates. The phylogenetic tree was clearly showed isolated of A. brassicae illustrated in dendogram framing three major cluster, in which Isolate A1 was completely different from rest of the isolates and made separate cluster. All other samples were also significantly different from each other i.e. A4 (78%), A5 (37%) and A2 and A3 (94%).
Alternaria brassicae, DNA, Fungal disease, Genetic variability, Oilseed crop, PCR, RAPD
