1Department of Nutrition and Food Science, Faculty of Agriculture, University of Samarra, Iraq
2Department of Nutrition and Food Science, Faculty of Agriculture, Mutah University, Iraq
3Department of Nutrition and Food Science, Faculty of Agriculture, Tikrit University, Iraq
*Corresponding Author: Nazieh Al-Khalaileh, Department of Nutrition and Food Science Jordan, Faculty of Agriculture, Mutah University, Iraq, Email: nazieh@mutah.edu.jo
Antimicrobials are agents that kill or prevent the growth of microorganisms like fungi, bacteria, viruses, or parasites. These agents (bioactive compounds) are obtained from plants. Incorporated into foods to extend their shelf life by inhibiting the effectiveness corruption microbe.
Antimicrobials were extracted from date kernel powder by soaking them in cold water, hot water and ethanol alcohol for 24 and 48 h. The effectiveness of antimicrobials against the growth of a number of microbes was evaluated by measuring the inhibition zone diameter (IZD) and minimum inhibitory concentration of antimicrobials (MIC) by using Well diffusion method.
Antimicrobial extracts by ethanol showed high inhibition with an average inhibition zone diameter of 12.35 mm, which is higher than the inhibition zone diameter resulting from antimicrobials extracted by hot water (6.03 mm) and cold (2.74 mm). The minimum inhibitory concentration (MIC) of the produced antimicrobials that was able to inhibit the growth of Klebsiella pneumoniae, Staphylococcus aureus and Fusarium oxysporum was 10 mg/mL and the corresponding inhibition zone diameters were 10.46, 10.03 and 11.00 mm, respectively. The growth of Candida albicans and Aspergillus niger was inhibited at concentrations of 2.5 and 5 mg/mL respectively, with inhibition zones ranging from 6.92 to 11.00 mm.
Date kernel powder, Inhibition zone diameter, Minimum inhibitory concentration
