1Department Plant Protection, Faculty of Agriculture, University of Kufa, Najaf, Iraq
*Corresponding Author: isam Adnan Radhi Aljuaifari, Department Plant Protection, Faculty of Agriculture, University of Kufa, Najaf, Iraq, Email: wisam.aljuaifari@uokufa.edu.iq
Meloidogyne javanica is a plant-pathogenic nematode. In many parts of the world, it is a serious agricultural pest. PCR-based sequencing of nematode mtDNA remains challenging because genomes are frequently organized and sequence similarities among lineages are low.
Using next generation sequencing (NGS), the genome of nematode-infected okra was sequenced, including the mitochondrial genome. The Illumina platform produced high quality 246,065,254 reads. The whole genome sequencing (WGS) was performed on okra at a high sequencing depth, approximately 31X coverage. The assembled reads against mitochondrion reference genome were 75,174 reads that completely covered the sequence with 96.5% identity.
Meloidogyne javanica mitochondrion isolate Kufa-Abbasiya consensus sequence of 17,654 nt length deposited in GenBank (accession number OR038715). Phylogeny revealed that the examined mitochondria genome was closely related to M. javanica and M. incognita isolated from USA. However, the alignment shows variation within repeat region.
Meloidogyne javanica, MtDNA, Okra plant, Next generation sequencing
