The Indian Journal of Animal Reproduction
Open Access
  • Year: 2019
  • Volume: 40
  • Issue: 1

Addition of chloroquine diphosphate or ascorbic acid in jersey bull semen and subsequent evaluation with respect to post-thaw incubation time and conception rate

  • Author:
  • Madhumeet Singh, A. Sharma
  • Total Page Count: 3
  • Page Number: 24 to 26

Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Himachal Pradesh Agricultural University, Palampur-176 062

*madhumeet2004@gmail.com

Online published on 21 January, 2019.

Abstract

Total 36 ejaculates (6 from each Jersey bull) were used in the study and aliquots were added with Chloroquine diphosphate or Ascorbic acid or served as control. Semen straws from all the groups were analyzed at 0, 1 and 2 h post-thaw incubation at 37°C for percent viability, progressive motility, reaction to hypo-osmotic solution and acrosomal integrity. Following post-thaw incubation, an improvement (p<0.05) in all the evaluated semen parameters was recored in groups with semen additives compared to control. The conception rate was better (p<0.05) using semen fortified with ascorbic acid.

Keywords

Acrosomal integrity, Ascorbic acid, Chloroquine diphosphate, Conception rate, HOST