Production, purification and characterization of alkaline protease from agro industrial wastes by using Aspergillus terreus (AB661667) under solid state fermentation

Proteases are the most important industrial enzymes and accounts at least 25% of global enzyme production. In the present work, Aspergillus terreus (AB661667) was used for the production of proteases by solid state fermentation using varieties of agro industrial waste (wheat bran, rice bran, green gram husk and black gram husk). Among the all tested varieties, wheat bran produced the highest activity in 67.4U/mg while black gram husk produced lowest protease as 59.1 U/mg under solid state fermentation conditions. All the purified alkaline protease from Aspergillus terreus(AB661667) showed a single protein band with a molecular weight of 38 KDa on SDS-PAGE. The optimum pH for the protease production was found to be 8.5 and optimum temperature at 35°C. All the data suggest that the selected strain of Aspergillus terreus (AB661667) can significantly produce a protease enzyme from the wheat bran substrate.