An investigation of biodiversity of endophytic fungi associated with some medicinal plants

The present investigations of Endophytic fungi residing in medicinal plants have not been systematically characterized. In this study, we isolates from 5 medicinal plant species using traditional morphological methods. The colonization rate, isolation rate, and relative frequency of these endophytes were investigated. The relationship between the composition of endophytic fungi and the chemical constituents of host plants was also explored for the first time. The results showed that endophytic fungi from these medicinal plants exhibited high biodiversity, host-recurrence, tissue-specificity, and spatial heterogeneity. Taxa of Colletotrichum spp, Aspergillus spp, Bispora spp., Geotrichum spp., Trichoderma spp. Cladosporium spp. Aspergillus spp. Gliocladium spp., Trichoderma spp.Fusarium spp. Trichosporoniodes spp Trichoderma spp and mycelia sterilia were the dominant fungal endophytes. Some Aliphatic, Aromatic, Amines, Aldehyde, Ester, Ketone and Phenol compounds were found to more likely coexist with certain endophytic fungi in the same plants. Our systematic investigation reveals that traditional medicinal plants are a rich and reliable source of novel endophytic fungi. This study was the first step towards understanding host-endophyte relationships based on the plant chemistry.