International Journal of Applied Science and Engineering
  • Year: 2014
  • Volume: 2
  • Issue: 2

Phytochemical Analysis and Antimicrobial Activity of Chlorophytum borivilianum against Bacterial Pathogen causing disease in Humans

1Dept. of Microbiology, Bidhannagar Govt College, Sector-1, Salt Lake City, Kolkata-700064, India

2Dept of Microbiology, Shri Venkateshwara University, Gajraula, Uttar Pradesh, 244236, India

*Email: rehanbiotech1@gmail.com

Online published on 10 June, 2015.

Abstract

The present communication deals with in vitro analysis of Antibacterial activity of extract of leaves and stem of Chlorophytum borivilianum Santapau and Fernandez (Liliaceae). The result of the preliminary investigation revealed the presence of alkaloids, glycosides nucleus, Saponins and tannins in leaves as well as in stem. The antimicrobial activity of leaves and stem extract of Chlorophytum borivilianum was studied against four bacteria among them two are Gram –ve bacteria (Escherichia coli and Klebsiella pneumonia) and two are Gram +ve positive bacteria (Staphylococcus aureus and Bacillus subtilis.) by agar disc cup diffusion method. Zone of inhibition produced by different extracts was tabulated. Only the aerial parts of plant inhibited the growth of bacteria at the concentration of 1000mg/ml and 500 mg/ml respectively. Extract Showed maximum antibacterial activity against all organisms tested in order of sensitivity as Staphylococcus > Bacillus subtilis > Klebsiella > Escherichia coli.

Keywords

Chlorophytum borivilianum, safed musli, leaves and stem extract, antibacterial activity, zone of inhibition