Characterization of antioxidant activity of streptomyces species isolated from shore regions of Kanathur, India

The aim of the research study concerns on screening, production and partial purification of antioxidant Secondary metabolites isolated from marine soil sample collected from Kanathur of Chennai, India. The sample was been serially diluted and plated on Starch Casein Agar and actinomycetes were selectively isolated. The broth culture of the ISP-2 medium was preferred for fermentation process and bioactive metabolites were been extracted using the solvent ethyl acetate. The crude compound (ABTRI 1 strain) was been screened for total antioxidant study and based on the activity other in vitro assays were carried out for crude compound. Secondary metabolites showed maximum inhibition at 100μg/ml, and the percentage of inhibition was been compared with the standard ascorbic acid. The partially purified crude compound was detected by Thin layer chromatography (UV and IODINE). The potential strain was characterized by 16s rRNA sequencing. The findings of the present study suggested that the marine isolate could be a potent natural source of antioxidants.