Screening of Soil Bacteria for Antibacterial Components

Antibacterial is a widely studied group of antimicrobials. Most of the antimicrobials use are from the bacteria. Bacteria are easy to isolate, culture, maintain and to improve their strain. In the present study 28 bacterial colonies obtained from various soil samples were isolated by Serial dilution and crowded plate method. Then these 28 colonies were selected for secondary screening of antimicrobial production. These mixed cultures were further purified by Discontinuous Quadrant Streaking to obtain pure cultures of bacteria. Antibiogram analysis was performed by Agar Well Diffusion Method against three test pathogens which. Escherichia coli, Staphylococcus aureus & Pseudomonas aeroginosa. Culture number MJPM2013 25 was observed with clear and larger Zone of Inhibition against all the pathogens.