Molecular Diversity Analysis in Groundnut Using Simple Sequence Repeat (SSR) Markers

In the present investigation eight genotypes of groundnut viz., Tirupati-4, TIR-25, ICGV-91114, TCGS-584, JL-220, ICGV-99029, K-1375 and TCGS-647 have been selected and analyzed with 45 SSR primer pairs to know the variation at DNA level. The results indicated considerable genetic variation among the genotypes. The results also revealed SSRs ability in detecting polymorphism among the genotypes screened. Among 45 SSR primers tested on the eight genotypes, ten primer pairs produced clear polymorphic bands. The results clearly showed that the genotypes have considerable levels of genetic diversity detected by SSR markers. In general, the genotypes analyzed showed wide genetic variation at DNA level.