Institute of Biotechnology, G B Pant University of Agriculture & Technology, Patwadangar, 263128, District Nainital, Uttarakhand, India
*Corresponding Author: E Mail: joshi.ankita11@gmail.com; Mob.: 0989757019
Online published on 28 February, 2017.
Present study is to evaluate the anticancer property of methanolic, ethanolic and aqueous extracts of Centella asiatica (Brahmi) in BHK-21 cell lines. Aqueous extract of C. asiatica leaves was prepared as cold extraction method. Methanolic (MeOH) and ethanolic (EtOH) extracts were prepared in Soxlet's apparatus and dried in vaccume evaporator. The BHK-21 cells were maintained in Animal cell culture laboratory of the institute in GMEM media supplemented with 5% fetal bovine serum as per standard procedures. The cells were treated with each of the extracts in triplet wells of cell culture 24 well polysterene plates and then allowed to grow for 24 hrs. The cell growth was inhibited within 24hrs; ethanolic and aqueous extracts showed better response. On microscropic examination clumping of cells, degeneration, rounding and shrinkge of cells were observed. The concentration of 100mg/ml of ethanolic extract showed high anticancer activity.
Centella asiatica, BHK-21 Cells, Anticancer Activity
