Department of Biology, College of Science, AL-Mustansiriya University, Baghdad, Iraq
*Corresponding Author: E Mail: Address: raghadalshybany@yahoo.co.uk; raghadalshybany@ymail.com; Ph.: 0770003231
Online published on 28 February, 2017.
Inhibitory activity of Enterococcus faecium concentrated filtrate was studied against L20B. The isolate obtained from the faeces of a newborn baby and identified according to cultural characteristics and biochemical testes using Rapid ID-32 strep. Isolate was grown in De Man Rogosa and Sharpe (MRS) broth. Cell lines were examined microscopically after 24 hrs of exposure to the bacterial filtrate. The cytotoxic effect and the cytological changes of E.faecium filtrate was a dose-dependent manner. The lowest concentrations (2 ng/ml) show significance induction on cell viability. There was no cytotoxic effect on cell line treating with the lower concentration. While the higher concentrations (2, 0.2) mg/ml inhibited cell growth. The inhibitory rate were 36.17%, 35.46% respectively.
Enterococcus faecium Filtrate, L20B Cell Line, Cytotoxic Effect
