Evaluation of Antibacterial Properties of Acacia nilotica

Acacia nilotica is a medicinal plant known for various medicinal uses. The present study was conducted to evaluate the antibacterial properties of leaves, stem, twig and bark of Acacia nilotica extracted using various solvents like water (room temperature and elevated temperature), 80% methanol (room temperature and elevated temperature), 70% ethanol (room temperature and elevated temperature), acetone, ethyl acetate and hot mixture of solvents (methanol +ethanol). Extracts were used in a final concentration of 100 mg/ml for assessment of antibacterial properties against two gram negative bacteria namely Escherichia coli, Pseudomonas aeruginosa and a gram positive bacteria namely Staphylococcus aureus using agar well diffusion method. The standard antibiotic Tetracycline was used in a final concentration of 1mg/ml. Hot ethanolic extract of Acacia nilotica bark and hot mixture of solvent extract of leaves gave the maximum inhibitory zone among all the extracts prepared.