This paper shows some of the different supercritical fluids available for use in SFC, and discusses the advantages that SFC has over HPLC and GC and how these can provide benefits to the industries which adopt this technique. High performance specifications and unique functionality of chromatographic techniques. This leads to the origin of Supercritical Fluid Chromatography (SFC). It is a rapidly expanding analytical technique. The main feature that differentiates SFC from other chromatographic techniques is the replacement of either the liquid or gas mobile phase with a supercritical fluid mobile phase. It is considered a hybrid of GC and LC technique. It has a unique characteristic of analyzing thermo labile or non-volatile substances. The present article reviews the fundamentals, instrumentation and varied applications of supercritical fluid chromatography in the analytical arena. The different setups available for SFC and how they compare along with the mobile phases and solid phases which are currently used are highlighted within the paper.
Supercritical fluid chromatography, mobile phase, modifier, critical temperature, critical pressure, mass spectrometry
