Effect of temperature on antimicrobial activity of Annona squamosa extract; phytochemical screening of bioactive compounds

The effect of temperature on antimicrobial activity four different plant extract of Annona squamosa i.e. 100% acetone leaf, 100% chloroform leaf, 70% ethanol hot leaf and 100% chloroform hot leaf were studied during the course of this research. Antibiogram analysis of extracts at room temperature, 22°C, 50°C, 75°C and 100°C were carried out. All the extracts except 100% chloroform hot leaf showed increase in activity at 50°C. There was an increase in zone of inhibition from 16.5mm to 17.0, 17.0mm to 17.5 against Staphylococcus aureus and Escherichia coli for 100% acetone leaf extract. An increase from 15.omm to 15.mm was recorded against Escherichia coli for 100% chloroform leaf extract. Phytochemical screening revealed presence of many bioactive compounds such as terpenoids, flavonoids etc in the extracts.