A large number of bacterial alkaline amylase have application in different industrial sectors they are detergent industry, food industry, textile industry, paper industry. In the present study 8 bacterial isolates of were screened for amylase production nature. Isolate 08 showing maximum hydrolysis was tentatively named as Bacillus species based on cellular morphology. Isolate 08 was further used in the study to optimize the physiological-chemical conditions and was found to show optimum growth at 37°C, pH7 and stationary phase was obtained after 96 hours of incubation. After optimization of production media amylase production was carried out under submerged conditions in a Bioreactor (lark). Crude extract obtained after centrifugation of fermented broth was used to get the total activity (145.59U) of amylase by DNS assay.
Amylase, Starch, Bioreactor, Fermentation, Enzyme activity
