Evaluation of antimicrobial properties of Bacillus megaterium inhabiting marine environments

Antimicrobial metabolites are produced as secondary metabolite by microorganism, such compounds are widely distributed in nature, they play an important role in regulating the microbial population of soil, water and sewage. In the present investigation, 7 bacterial strains isolates from marine sediment, collected from the SAGAR VIHAR, SHANPADA MUMBAI (MAHARASTRA) found during primary screening (crowded plate technique) in which seven bacterial strain was found to be effective namely as MJSS 01 to MJSS 07 and MJSS 07 was selected for further strain improvement because its found most effective strain in secondary screening against the tested pathogens namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Strain improvement done by U.V. treatment and EtBr treatment of selected strain. Isolate was identified as Becillus megaterium on the basis of Bergey's manual. During the study of physiochemical factor of bacterial isolate MJSS 07 the maximum growth was observed at a temperature 37°C and a pH of 7.0. The production media was optimized for best Nitrogen, Carbon and Metal ions sources. A production of crude antibacterial metabolite was carried out by submerged fermentation and crude extracellular metabolites obtained after centrifugation was purified by solvent extraction method. Metabolites extracted by ACETONE gave the best zone of Inhibition 22.5 mm for E.coli respectively. Later on Dialysis was performed to check whether the antimicrobial extracted is proteinacious in nature or not and after that MIC was also done to check its potency.