1Senior Scientific Officerr (SSO), Biotechnology Division, BangladeshInsttitute of Nuclear Agricculture (BINA), Mymensingh, Bangladesh
2Chief Scientific Officer (CSSO) and Head, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh, Email: Chandra.mongal@gmail.com, Cell: +8801712725689
3MS in Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh. Cell: +8801719547179
4Upazillla Agriculture Officer (UAO), Ulipur, Kurigram, Bangladesh, Cell: +8801731502184
*Corresponding authorr: Bishnu Pada Ray: E Mail: bpray2010@gmaail.com; Cell: +8801710586093
Online published on 17 July, 2017.
The present investigations havve helped to establish clear-cut identity of all the cultivars under consideration which will be of great utility for the protection of Plant Brreeders’ Rights. Six markers (RM556, RM562, RMM594, RM7075, RM1287 and RM3843) weere used to evaluate BPR1 and BPR2 lines withh donor parent BRRI dhan36 by amplification of the DNA sequence to cold tolerance. The bands obtained BPR1 and BPR2 lines were compared to the band obtained from cold tolerrance BRRI dhan36. The BPR1 and BPR2 lines having similar banding pattern to BRRI dhann36 were considered as cold tolerant. So, colld tolerant QTL has been introduced to these twoo lines. The average growth duration of BPPR1, BPR2, BRRI dhan28 and BRRI dhan36 weere 149,148,148 & 145 days with the averagee yield of 6.50 t/ha, 7.10 t/ha, 7.20 t/ha & 6.10 t/hha respectively. According to all consideratioon, BPR2 produced highest yield (7.10 t/ha) andd cold tolerant performance which was higgher than the other genotypes. The objective of tthis study was to markers related to cold tolerance that can be used to facilitate marker-assiisted breeding and evaluation of genotypes in the cold prone environments under the managgement practices of researchers.
MUS, Extraction, Cold, QTL, Rice
