International Journal of Biotechnology and Allied Fields
  • Year: 2017
  • Volume: 5
  • Issue: 12

Mist drainage characteristics of spherical hairy root bed in nutrient mist reactor

  • Author:
  • SR Katuri, R Khanna
  • Total Page Count: 12
  • Page Number: 139 to 150

Department of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi, India-110016

*Corresponding Auuthor: E Mail: ksrao.iitd@gmail.com; Tel: +91-9642653815

Online published on 9 April, 2018.

Abstract

Nutrient Mist Reactor is the best bioreactor system to grow hairy roots in which gas phase nutrient is a continuous phasee. The mist captured by the growing root bed forms a layer over a root which eventually grows with culture time and resists oxygen transfer through it. Drainage of excess mist from the root beed characterized using linear model and loogarithmic model. The intermitent operation of NMR with linear drainage and logarithmic drainage is discussed extensively. The mist-ON cyycle duration is controlled by the liquid film thickness over roots which can be altered by feed flow rates and drainage rates. The mist-OF cycle duration is controlled by the nutrient concentration of the held liquid. Low flow rates and high drainage rates promotes oxygen mass transfer rates with careful monitoring of liquiid nutrients availability in the film.

Keywords

Miist deposition, drainage, hairy root, mass trransfer