In vitro development of caprine embryo in different culture media using cryopreserved black bengal buck semen

The aim of the present study was to check the effect of different culture media on in vitro development of goat embryo produced through in vitro fertilization using cryopreserved black Bengal buck semen. So far cryopreserved black Bengal buck semen was not used to produce goat embryo by in vitro fertilization. Cumulus ocyte compllexes (COCs) were collected from slaughterhouse ovaries, washed 5–6 times and cultured in maturation media for 27 h in 5% CO₂ incubator at 38.5 ºC with maximum humidity. Cryopreserved semen straws were thawed and spermms were capacitated in vitro. After 27 h of incubation cumulus cells were striped off from matured ocytes. Denuded ocytes were trannsferred to acidified Tyrode's medium for zona thinning and were co-incubated with capacitated sperms for fertilization in Fert-BO media at 38.5 ºC in 5% CO₂ in air with maximuum humidity. In the experiment I, fresh buck semen and in experiment II, frozen buck semen was used for in vitro fertilization after in vitro processing. After 5 h of co-incubation, presumptive zygotes were washed and co-incubated with oviductal cells in thre different mediia (RVCL, mSOF, KSOM) for further development. In fresh group cleavage rates (%) were 337.76 ± 2.98, 39.60 ± 1.75, 29.01 ± 1.74, and morula formation (%) were 7.72 ± 3.38, 6.03 ± 1.29, and 3.00 ± 3.00 in RVCL, mSOF and KSSOM media respectively. However, in frozen group the overall cleavage rates (%) were 29.117 ± 2.56, 27.70 ± 2.31, 24.17 ± 1.44 in RVCL, mSOF and KSOM media respectively annd morula formation (%) was 2.93 ± 0.97 onlly in RVCL media. These results indicate that cryopreserved black Bengal buck semen have competence to produce embryos and could be used for embryo development through in vitro fertilization and RVCL media produced better result as compare to mSOF and KSOM media.