1Department of Biotechnology, Marathwada Agricultural University, Parbhani, Maharashtra India.
2Virology Laboratory, Division of Plant Pathology, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore-560089 India.
3Department of Biotechnology, Institute of Science, Aurangabad, India.
Cucumber mosaic virus (CMV) causing mosaic and chlorosis of banana in Marthwada region was characterized on the basis of biological, serological and RT-PCR amplification of Coat protein. Mechanical inoculation tests, the virus was found to infect members of chenopodiaceae, Cucurbitaceae, Fabaceae, and Solanaceae. Partially purified virus preparations revealed the presence of isometric particles of 28nm in the electron microscopy. Insect vector transmission of CMV has indicated that Aphis gossypii was more efficient than A. craccivora in transmitting CMV in a non persistent manner. Using DAS ELISA CMV could be efficiently detected in different banana sample which are both symptomatic and symptom less plants. This would help in development of diagnostic tool for identifying and certifying planting material to check spread of disease.
Banana, Chlorosis disease, Cucumber mosaic virus, ELISA detection, RT-PCR amplification
