This study was conducted in order to follow the expression of two important genes IGF-R and FSHR that related to the fertility of bovine during the maturation stage of oocytes (COC development). We have studied the expression of these two genes in both immature and mature oocytes by isolation of cDNA directly without extraction of RNA by using small number of cells as a rapid method. In addition, we have isolated these two genes from blood sample as a different source of donor cells via RNA extraction. Our results showed that IGF-1R and FSHR genes are expressed clearly in the mature oocytes if compared with the immature oocytes and these two genes are clearly expressed inside mature oocytes itself. After the isolation and identification by PCR and the purification we have cloned the FSHR gene by using pUCm-T vector and have used PMD18T-simple vector to clone IGF-1R then we make sequencing for these two genes which were isolated from the germ cells. These two genes will be ready for transfection and transfer to other type of cells.
Gene expression, FSHR, IGF-1 R, fertility, cell cloning, bovine
