Rapid micropropagation of Aloe vera L. via shoot multiplication

In this research, shoot tip explants of Aloe vera L. were used for rapid proliferation. The present study indicates the importance of biotechnology investigations in order to overcome limitations such as slow growth, to be expensive and low income practice in this plant.

This study can be utilized to extend a manner to rapid micropropagation of plants that may provide a proper source for medical and cosmetic industries.

Explant used for the in vitro culture was shoot tip. The shoot tip explants was disinfected with 2% NaOCl and washed throughly with sterile water. Then, explants was placed on soild MS medium with the addition of various concentration of benzyladenine and α-naphthaleneacetic acid. After 8 weeks the best proliferation of shoot per explant (9.67) and the best rooting was shown on the medium supplemented with 0.5 mg/l BA + 0.5 mg/l NAA. The rooted plantlets were gradually acclimatized in a plastic pots containing a mixture of cocopeat and perlite (1:1) covered with transparent plastic. About 95% of the transplanted plantles survived.