International Journal of Biotechnology & Biochemistry
  • Year: 2008
  • Volume: 4
  • Issue: 2

Effect of different hormonal combinations on in vitro propagation of rosemarinus offcinalis

  • Author:
  • Iffiat Fayaz Malik, Mohd. Altaf Wanil, A.S. Shawl, P.W. Ramteke
  • Total Page Count: 8
  • Page Number: 161 to 168

Division of Plant Breeding and Genetics, SKUAST-K, Shalimar, Srinagar (J & K), India-191 121.

Abstract

Effect of different hormonal combinations on invitro propagation of Rosemarinus offcinalis was investigated using shoot tips and nodal segments as explants. Surface sterilization of shoot tips and nodal explants was carried out by using HgCl2 (0.1%) for one minute and one and half minute respectively. All the cultures were maintained at 22±2°C and 16 hour photoperiod. Green, friable callus formation in both nodal and shoot tip explants of Rosemarinus officinalis on MS media supplemented with BAP (200μM) and IAA(100μM), NAA (150μM) and IAA (100μM) respectively was observed in the present study. Maximum shoot multiplication from shoot tips and nodal segments was achieved on MS medium augmented with BAP (100μM) + IAA (100μM) and BAP (20μM) respectively. Best rooting (85%) was observed in MS half salt strength media BAP

6-benzylaminopurine

2, 4-D

2, 4-dichlorophenoxyacetic acid

HgCl2

Mercuric chloride

IAA

Indole acetic acid

IPA

Indole propionic acid

MS

Murashige and Skoog (1962)

NAA

Naphthalene acetic acid

Keywords

Rosemarinus officinalis, In vitro propagation, Labiatae, Rosemary oil, Shoot regeneration