Production and enzyme activity of an extracellular protease from aspergillus niger nd bacillus subtilis

An extracellular protease enzyme production from Aspergillus niger and Bacillus subtilis in culture medium supplemented with glucose and trisodium citrate as a substrate. These microorganisms could grow up to 60°C within a broad pH range of 5 to 10 with an optimal growth temperature and pH at 60°C and 8.0 respectively. For the three Bacillus subtelis was shown better growth at temperature 50°C and pH 9. Similarly Aspergillus niger was well cultured at temperature 60°C and pH 8. Maximum protease enzyme activity was recorded at temperature 60°C (26.1±0.07 Uml⁻¹min⁻¹) and pH 8 (26.0±0.07 Uml⁻¹min⁻¹). The results showed that Bacillus species under study are good producers of extracellular protease at high temperature in alkaline medium.