Phospholipids Assay of Daphnia Carinata Using 1-D and 2-D Nmr Techniques

Daphnia carinata has been extensively used in larviculture. Phospholipids are extremely critical for the larval growth. Phospholipid fraction of Daphnia carinata was evaluated by NMR technique. An expanded part of proton NMR spectrum of the phospholipids fraction showed that the head group of Phosphatidylcholine (PC) was assigned as δ = 4.09 [C(1)H₂] and 3.76 [C(2)H₂] ppm with comparable intensities of 47.74 and 48.34 for respective signal. The head group of Phosphatidylethanolamine (PE) was designated as δ = 4.06 [C(1)H₂] and 3.66 [C(2)H₂] ppm with intensities of the signals 50.98 and 50.31, respectively. δ = 4.25 [C(1)H₂] and 3.87 [C(2)H] ppm were assigned to the head group of Phosphatidylserine (PS). The intensities of the signals at δ = 4.25 and 3.87 ppm were 108.46 and 45.02, respectively. In ¹H-NMR spectrum, the head group of Phosphatidylinositol (PI) was assigned as δ = 4.38 [C(1)H], 4.14 [C(2)H], 3.9 [C(3)H], 4.3 [C(4)H], 3.97 [C(5)H] and 3.78 [C(6)]. The almost comparable intensities were 41.62, 36.49, 34.87, 41.34, 32.68 and 43.94, respectively. The 2-D homonuclear double quantum filtered correlated spectroscopy and 2-D Total Correlated Spectroscopy confirmed these results. The relative amount of PE, PC, PS, PI in the phospholipid fraction of Daphnia carinata were as follows: PC: PE: PS: PI = (47.74/2): (50.98/2): (108.46/2): (41.67) = 1: 1.06: 2.27: 1.74.