1Al Neelain University, Faculty of Science and Technology, School of Life Science, Khartoum, P.O. Box: 12702, Code: 11121, Sudan.
Abbreviations2,4-D
2,4-dichlorophenoxy acetic acid,
IAAIndole-3-acetic acid,
NAAα-Naphthalene-3-acetic acid,
BAP6-Benzylaminopurine),
MSMurashige and Skoog basal medium.
The effect of three Auxins (2,4-D, IAA and NAA) and two cytokinins (BAP and Kinetin) alone or in combination on in vitro callus induction and plant regeneration of a Sudanese cultivar of tomato (Solanum lycopersicon Var. Alla karim) was studied.
Results show that there are significant differences (P< 0.05) between different growth regulators used, in term of callus induction and regeneration. Control and all Auxins except 2,4-D at all concentrations used (0.1, 0.2, 0.5, 1.0, 1.5 mg/l) were not able to produce calli. All cytokinins were able to produce high percentage of callus induction as well as all combinations of auxins with the cytokinin. BAP at 2.0 mg/l produced high percentage of callus induction and among all media used in this study it also produced the highest number of shoot regeneration (4.67 shoot per explant).
Among the different rooting media used in this study, results showed that full strength MS medium when fortified with NAA at 2.0mg/l produce the highest number of regenerated roots (14.5 roots per explant).
callus induction, Auxins, Cytokinin, explants, Growth Hormones
