Institute of Clinical Medical Science, Section of Immunology Lund University, BMC I:13, SE-221 84 Lund, Sweden.
* Correspondence
Objectives: To test the hypothesis that in vivo oxidation of serum protein thiols can be used to estimate other clinical compromising events going on in the body such as the effectiveness of DNA repair and lifestyle-induced clinical responses.
Design and Methods: (i) Serum protein thiols were quantified in 200 μl blood serum after treatment with saturated ammonium sulfate to give 80% of the saturated level and then further treatment with the colorimetric agent 5,5-dithio-bis-(2-nitrobenzoic acid (DTNB). (ii) A self-report clinical index score was created and administered to participants to simultaneously evaluate appetite, pain, fatigue, self-perceived health status, and energy for this purpose. (iii) A crossover trial design (i.e. before and after treatment) was used to further evaluate the clinical effectiveness of quinic acid ammonium chelate to enhance DNA repair.
Results: It was shown that enhanced repair of DNA using the surrogate estimate of serum thiols correlated by linear regression analyses to the degree and duration of the psychotropic-sensitive lifestyle clinical responses being evaluated(p < 0.02).
Conclusions: A cheap and rapid biochemical method sensitive to estimating anti-aging effects of lifestyle criteria in clinical material and in blood serum is proposed.
Quinic acid, clinical response, DNA repair, oxidative stress, serum protein thiol
