International Journal of Biotechnology & Biochemistry

  • Year: 2010
  • Volume: 6
  • Issue: 2

Increasing of Biodesulfurization Activity of Newly Recombinant Pseudomonas Aeruginosa ATCC 9027 by Cloning the Flavin Reductase Gene

  • Author:
  • Jamshid Raheb1,, Mohammad Javad Hajipour1,2, Babak Memari1
  • Total Page Count: 11
  • DOI:
  • Page Number: 219 to 229

1National Institute of Genetic Engineering and Biotechnology (NIGEB), Karaj Tehran highway, Pajoohesh Blvd. P.O. Box. 14155-6343.

2Department of Biological Science, Imam Hossein university, Tehran - Iran.

Abstract

Sulfur emission through fossil fuel combustion is a major cause of acid rain and air pollution. The main aim of running of this research was increasing the biodesulfurization activity of recombinant P. aeruginosa ATCC9027 by cloning of flavin reductase gene. There are four gene dszA,B,C,D which are involved in desulfurization pathway (4s) and allowed release of sulfur after four enzymatic steps. In this pathway Monooxygenases DszC and DszA enzymes require free FMNH2 which provided by DszD enzyme. In the present study the pVLT31 vector harboring flavin-oxidoreductase gene (dszD) was transferred into the recombinant P. aeruginosa ATCC9027 which contained dszABC gene in its chromosome stably. We found that the biodesulfurization acivity of the recombinant P. aeruginosa ATCC9027 enhanced when these four genes co-expressed. The data obtained here confirmed by biochemical quantitative Gibbs assay.

Keywords

Pseudomonas aeruginosa ATCC9027, Dibenzothiophen, Gibbs assay