International Journal of Biotechnology & Biochemistry

  • Year: 2010
  • Volume: 6
  • Issue: 3

Homo Sapiens DCC Gene: In Silico Analysis to Understand the Affect of Mutations in Colon Cancer

  • Author:
  • Siddiqui M. Asif1, Amir Asad1, Anjali S. Malik1, Arvind Arya1, Neelesh Kapoor1, Hirdesh Kumar1
  • Total Page Count: 8
  • DOI:
  • Page Number: 419 to 426

1Department of Biotechnology, Meerut Institute of Engineering and Technology, N.H. 58, Delhi-Roorkee Highway, Baghpat Road Bypass Crossing, Meerut-250005, UP, India.

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Abstract

Deleted in Colon Cancer (DCC) is a tumor suppressor gene, which is deleted in 70% of Colon cancers. Here, we investigate the protein product of DCC in much detail towards a better understanding how it leads to cancers. The protein sequence was retrieved through NCBI. Three mutations in protein sequence have been identified that are associated with colon cancer. Domain analysis shows that all the three mutations are present in functional domains. It was concluded from the results of physicochemical and structural analysis that the change of a single amino acid disrupts the structure of domain that leads to tumorigenesis. The analyses were performed using various in silico methods.

Keywords

Deleted in Colon Cancer (DCC), NCBI, BLASTP, Multiple Sequence Alignment (MSA), PROTPRAM and SOPMA.