Light is a very important environmental factor which affects plant growth and development in various important ways. Not only quality of light but quantity of light is also very important in determining the growth of plants therefore the present study was aimed to analyze the growth of wheat seedlings in different light qualities. Wheat seedlings grown on germination paper under high intensity red light (400 μmoles m−2 s−1) were yellowish-white in color and accumulated minimum amounts of chlorophyll and carotenoid. However wheat seedlings when grown in dark were etiolated and when grown under different light conditions, as high intensity red light in vermiculite, red +blue, far-red, red + far-red, and white light were green in color and accumulated different amounts of chlorophyll and carotenoids. 2D-PAGE of total protein isolated from plants grown under different light regimes showed absence of many proteins in red light grown non-green seedlings and etiolated seedlings as compared to green seedlings. All the green plants grown under different light conditions showed steady state expression of many proteins. These seedlings also accumulated many light quality specific proteins, i.e., those proteins were only present in a particular light environment and absent in the other. Western blot analysis of Rubisco LSU, OEC33, and Cyt-f showed reduced abundance in red light grown non-green seedlings but were abundantly present in green seedlings. POR was accumulated in etiolated seedlings and reduced in other light grown seedlings. The results show that light quality is a very important environmental factor in influencing chlorophyll content as well as expression of other protein and is an important factor affecting plants growth under different areas receiving different qualities of the light.
Chlorophyll, carotenoid, light quality, protochlorophyllide oxidoreductase, photomorphogenesis
