Micropropagation of Pongamia pinnata (L.). Pierre- a Native Indian Biodiesel Tree from Cotyledonary Node

The cotyledonary nodes were used as explant for developing a regeneration system for Pongamia pinnata (L.). In vitro raised seedlings were used for the production of multiple shoots on MS medium supplemented with 8.8 μM Benzylaminopurine (BAP) and 0.53 μM Naphthalene acetic acid (NAA). Shoot cultures were established and maintained without loss of vigor by repeated sub-culturing. Thus, from a single cotyledonary node, about 16–24 shoots were obtained. Shoots formed in vitro were rooted on full strength MS medium supplemented with different concentration of Indole-3-butyric acid (IBA). Plantlets were successfully established in vermiculate for hardening.