Effect of Frequency of Subculturing of different Isolates of Beauveria bassiana Vuillemin on their Biological Properties

An experiment was conducted to find the effect of repeated subculturing on biological properties viz., radial growth, sporulation and conidial viability of four strains (Bb-13, Bb-11, Bb-5A and Bb-N) and two local isolates (Bb-L-1 and Bb-L-2) of Beauveria bassiana Vuillemin on synthetic media. The results of the experiment showed no significant reduction of the biological properties during the initial 1^st to 7^th subculturing but showed significant negative effects on the biological properties after 7^th subculturing. Molecular characterization of the strains using PCR based RAPD indicated considerable variability among the isolates.