1Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641003), India
2Department of Rice, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641003), India
*Correspondence to E-mail: kumaravadiveln@yahoo.com
Online published on 31 March, 2014.
In rice, RILs were screened to assess the resistance to brown plant hopper based on the standard seedbox screening test (SSST) in the greenhouse. The parents viz., IR50 and Rathu Heenati had the mean score damage of 5.75 and 3.08, respectively. For the RILs, the leaf damage score ranged from 3.0 to 9.0. As many as 15 SSR primer pairs belonging to chromosome 3 were used to screen the parents for polymorphism. This region was previously reported to be associated with BPH resistance. A total of five polymorphic markers were identified between IR50 and Rathu Heenati. The polymorphic markers were surveyed on 133 RILs to establish their segregation pattern. Out of the five marker loci studied, three marker loci viz., RM520, RM3180 and RM6283 showed the expected segregation ratio of 1:1 based on the χ2 test at five per cent level. The SSR marker RM2346 was identified to be associated with the BPH resistance based on the damage score from SSST. The SSR marker RM 2346 identified in the present study may be useful in marker-assessed select program after further testing, towards the development of new rice lines with enhanced level of resistance to BPH
Rice, Nilaparvata lugens, standard seed box screeing (SSST)
