Effect of Culture Media on In-vitro Regeneration of Anthurium (Anthurium andraeanum Lind.) from Axillary Bud Explants

Direct regeneration of anthurium from adventitious bud explant was studied. Half-MS medium, supplemented with coconut water 15% (^v/_v), NAA (1 mg l⁻¹) and BAP (3 mg l⁻¹) was found the most effective medium regarding percentage of explant survivability (84.45%), earliness in shoot production, and number of shoot and leaf production culture⁻¹ at the time of first transfer. The other media with less cytokinin also showed effects that were less favorable as compared to this medium. Explants on growing media having MS+NAA (0–1 mg l⁻¹)+BAP (0–3 mg l⁻¹) and ¹/₂ MS+coconut water (15% ^v/_v)+NAA (1 mg l⁻¹)+BAP (0–1 mg l⁻¹) failed to survive and proliferate. Results revealed that increase in the concentration of BAP pronounced the effect of the medium. ½ MS medium supplemented with coconut water 15% (^v/_v), NAA (1 mg l⁻¹) and BAP (3 mg l⁻¹) was effective for shoot regeneration of anthurium from adventitious bud explants. For early rooting ½ MS medium supplemented with NAA (1 mg l⁻¹) and IBA (2mg l⁻¹) was found ideal. This medium (AR₂) started rooting on 39.10 days after culture. Though culture on ½ MS medium supplemented with NAA (1mg l⁻¹)+IBA (3 mg l⁻¹) started rooting 42.67 days after transfer but this medium (AR₃) represented 84% explant survivability hence, may be advocated for rooting of anthurium micro-cuttings.