1PG Student, Dept. of Biochemistry, KS Hedge Medical Academy, Mangalore
2Professor & HOD, Dept. of Biochemistry, KS Hedge Medical Academy, Mangalore
3Lecturer, Dept. of Biochemistry, KS Hedge Medical Academy, Mangalore
4Professor, KS Hedge Medical Academy, Mangalore
Reference Intervals are the most common decision making tools for interpretation of various biochemical reports. Age, sex, ethnicity, diet, physical and socio-economic conditions, affect the physiology of population. Hence it is necessary to setup reference values that are applicable to specific population rather than to borrow them from other population based studies or from literature from reagent kit manufacturers. So we intend to establish the reference intervals of serum bilirubin profile in southern coastal Karnataka population.
Hospital based cross sectional study was done involving 200 subjects. The subjects were randomly selected from the population, which included medical students, health professionals, and those who attended different OPDs and health camps organized by the K S Hegde hospital for general check-ups under health plan scheme. Total bilirubin and direct bilirubin was estimated by dichlorodiphenyl diazonium method. Indirect bilirubin (Total bilirubin-Direct bilirubin) was calculated.
The reference intervals observed for men and women are as follows: Total bilirubin (0.1–1.3)mg/dl and (0.1–1.38)mg/dl, serum direct bilirubin (0–0.5)mg/dl and (0–0.3)g/dl, and serum indirect bilirubin (0.1–0.9)mg/dl and (0.1–1.3)mg/dl respectively. With the advancement of age, a difference in the reference interval was observed among both the sex.
The present study showed a difference in the reference intervals between males and females and also a categorical age wise difference was obtained.
Bilirubin, Gender, Reference interval, Healthy population, Age
