Department of Microbiology, Sri Sathya Sai Med. College and Research Centre, Ammapetai, Kanchipuram Dist. Tamilnadu
The larvae of strongyloides stercoralis were obtained from larvally contaminated fresh Coriander plants by modified Baermann's method. The recovered larvae were introduced into petridish, containing 5–7 young stems with roots, 5-10ml. of clean tap water, 10 micrograms of Ampicillin. Incubated at room temperature with lid of petridish covered with cellophane tape, noting initial type of larvae and their approximate number, maintaining the moisture throught. The preparation was monitored for increase in the number of larvae and type of larvae by periodical observation under microscope. We could note the increase in the numbers of all types of larvae, varying in proportion to one another, as culture progressed up to 4–5 weeks. Larvae could be separated by simple centrifugation and can be used for research purpose without using laboratory animals as source of larvae for studies. The larvae were identified by their morphology. The technique is very simple and cost effective.
