1Associate Professor, Department of Biochemistry, S Nijalingappa Medical College, Bagalkot, Karnataka
2Assistant Professor, Department of Biochemistry, S Nijalingappa Medical College, Bagalkot, Karnataka
3Professor, Dept of OBG, S Nijalingappa Medical College, Bagalkot, Karnataka
4Post graduate, Department of Biochemistry, S Nijalingappa Medical College, Bagalkot, Karnataka
*Corresponding author: Sunitha Pujar, Associate Professor, Department of Biochemistry, S Nijalingappa Medical College, Bagalkot, Karnataka. Ph. No.: 08123187085, Email: drsunitapujar@gmail.com
Online published on 31 January, 2015.
Diabetes is a chronic metabolic disorder that is often associated with unacceptably high disease burden especially in developing countries. The persistent hyperglycemia causes increased production of free radicals, can also induce oxidative stress and become the main factor for predisposing the complications in diabetes, including neuropathy. Diabetic neuropathy can be directly related to oxidative damage and change in the antioxidant defences of the nerve cell. Oxidative stress appears to be primarily due to nerve ischemia and hyperglycemic auto-oxidation. The study was designed to estimate status of lipid peroxidation product i.e. malondialdehyde and antioxidants reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and ascorbic acid (vitamin C) in patients with type 2 diabetes mellitus with and without neuropathy and compare them with healthy controls.
The study included 50 patients of type 2 diabete mellitus without any complication, 50 patients of type 2 diabetes mellitus with neuropathy and 50 age and sex matched healthy individuals served as controls. Fasting and postprandial venous blood sample was analysed for malondialdehyde, fasting blood glucose, reduced glutathione, glutathione peroxidase, glutathione reductase, catalase, superoxide dismutase, ascorbic acid (vitamin C) and postprandial blood glucose.
The serum MDA, FBS, PPBS and CAT levels were significantly elevated in type 2 diabetics without neuropathy compared to healthy controls (p?0.001). The serum MDA, FBS, PPBS and CAT levels were significantly elevated in type 2 diabetics with neuropathy compared to type 2 diabetics without neuropathy (p?0.001). There was significant decrease in the levels of GSH, GPx, GR, SOD and vitamin C in type 2 diabetics without neuropathy compared to healthy controls (p?0.001). There was significant decrease in the levels of GSH, GPx, GR, SOD and vitamin C in type 2 diabetics with neuropathy compared to type 2 diabetics without neuropathy (p?0.001).
Diabetic patients with neuropathy have lower levels of antioxidants. This low value may be due to oxidative stress caused by hyperglycemia that reduces the concentration of the antioxidant status of the body.
Diabetes Mellitus, Neuropathy, Antioxidants, Malondialdehyde, Oxidative Stress
