1Post Graduate, Department of Microbiology, Mediciti Institute of Medical Sciences, Ghanpur Village, Medchal Mandal, R.R Dist, Andhra Pradesh
2Associate Professor, Department of Microbiology, Ghanpur Village, Medchal Mandal, R.R Dist, Andhra Pradesh
3Professor and Head of the DepartmentMediciti Institute of Medical Sciences, Ghanpur Village, Medchal Mandal, R.R Dist, Andhra Pradesh
*Corresponding author: B A Ankitha, Post Graduate, Department of Microbiology, Mediciti Institute of Medical Sciences, Ghanpur Village, Medchal Mandal, R.r Dist, Andhra Pradesh, 501401, E-mail- ankithabhusarapu@gmail.com, Ph. No. 9700629875
Online published on 15 October, 2015.
Septicemia in neonates refers to generalized bacterial infection documented by positive blood culture in the first four weeks of life and is one of the leading causes of neonatal mortality and morbidity in India. AIM: To isolate and identify the bacterial etiologic agents responsible for neonatal sepsis and to determine the susceptibility Pattern of isolates in a tertiary care hospital in Hyderabad.
One hundred blood samples were collected from patients and processed in accordance with standard protocols. Antibiogram of the isolates was done by disc diffusion method according to CLSI recommendations.
Blood culture reports were positive in 50% of the cases. Out of the total positive cases, early onset sepsis was present in 64% cases and late onset sepsis was observed in 36% of the cases. Best overall sensitivity among gram negative isolates was to imipenem(93%) followed by amikacin(87%) and piperacillin/tazobactam(87%). Gram positive isolates had sensitivity of 100% to vancomycin, 100%toamoxiclav, 82% to piperacillin/tazobactam.
Gram negative organisms of Pseudomonas, Klebsiella and gram positive organisms of Coagulase negative staphylococci were the leading causes of neonatal sepsis in this study and most of them were resistant to multiple antibiotics.
Blood culture, neonatal sepsis, pseudomonas species
