Bacteriological Profile of micro-organisms and their Antibiotic Susceptibility Pattern in Pus Samples

Pyogenic infections are infections caused by pus producing bacteria. In recent years wound infection particularly due to multidrug resistant pathogens are increasingly being encountered in hospital settings. Antimicrobial resistance has emerged as a major public health problem all over the world. Resistant microbes cause wound infection which leads to failure of treatment, longer stay in hospital and increases the cost of treatment.

To find Bacteriological profile of micro-organisms and their antimicrobial susceptibility pattern in pus samples to formulate the antibiotic policy for empirical treatment of patients.

150 samples of pus were processed in Clinical Microbiology Laboratory, Santosh Medical College and Hospital, Ghaziabad. The samples were cultured on Blood Agar, Nutrient Agar and Mac Conkey Agar plates. The plates were incubated aerobically at 37°C for 24 hours. The isolates were identified by conventional method. The antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion method. Data were collected and analyzed by using appropriate statistical methods.

Out of 150 pus samples, 62 were found culture positive. The prevalence of wound infection was found 41.3%. Among gram positive cocci Staphylococcus aureus was found most common organism and among gram negative bacilli Klebsiella pneumoniae followed by Pseudomonas aeruginosa were found the commonest organism. The Gram positive cocci were found resistant to Penicillin, Ampicillin, Amoxicillin Clavulanic Acid and Erythromycin, sensitive to Linezolid, Vancomycin, Ceftriaxone and Clindamycin. Gram negative bacilli were found resistant to Cotrimoxazole, Ciprofloxacin and sensitive to Amikacin, Piperacillin Tazobactum, Meropenem and Imipenem.

This antimicrobial susceptibility testing and antibiogram of isolates can provide a knowledge about to start correct empirical treatment of patient till culture results are available.