Comparative efficacy of three serological tests for antigenic characterization of fmd virus type ‘o’ isolates

Three serological tests viz. micro complement fixation test (mCFT), soundwich enzyme-linked immunosorbent assay (sELISA) and liquid phase blocking ELISA (LPBE) were used to compare the r value for characterization of 10 isolates of FMD virus type O from field outbreaks with the IVRI vaccine virus strain. In unidirectional testing, the r value of most of the isolates was highest with sELISA and the lowest with the mCFT in unidirectional testing. In the reverse direction testing, the r value of the reference virus strain was almost same with the three tests except the lower r value of the reference strain with two of the isolates antisera in LPBE and with one of the isolate in mCFT.